Flow Injection Tutorial

Changing the flow velocity is the most powerful way of

creating a local turbulence, thus breaking laminar flow

pattern and redistributing the parabolic flow profile. This

can be achieved by

Until recently, flow injection, utilized only the first two

tools. In the confluence point construct (A) the stream

carrying the sample zone merges with reagent zone.

If the flow rates of sample and reagent streams are equal

and the geometry of the mixing point is correct, instant

mixing takes place (B) due to local turbulence. (See video

clips on the next page). Flow programming now also

applied to FI allows further optimization of the assay

protocol.

Sequential Injection is a single stream technique and

therefore relies on the change of flow geometry and

flow programming to promote mixing of sample and

reagent zones.